Design and synthesis of a PD-1 binding peptide and evaluation of its anti-tumor activity — ASN Events
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  3. Design and synthesis of a PD-1 binding peptide and evaluation of its anti-tumor activity

Design and synthesis of a PD-1 binding peptide and evaluation of its anti-tumor activity (#182)

Abdul Baset Abbas 1 , Bingjing Lin 1 , Peiwei Yang 1 , chen Liu 1 , Arwa Morshed 1 , Jialiang Hu 1 , Hanmei Xu 1
  1. China Pharmaceutical University, Nanjing, Jiangsu, China

We have designed and synthesized a new peptide FITC-YT-16 to target PD-1. MST revealed that FITC-YT-16 interacted with PD-1 at a Kd value of 17.8 ± 2.6 nM. T cell imaging and flow cytometry revealed high affinity of FITC-YT-16 to PD-1. Then the peptide was tested for its ability to enhance T cell activity against tumor cell lines, including TE-13, A549, and MDA-MB-231.We also finded that FITC-YT-16 efficiently blocked PD-1 signaling pathways and promoted T cell inflammatory responses by elevating IL-2 and INF-γ levels. Moreover, FITC-YT-16 has the ability to activate T cell cytotoxicity. Therefore, FITC-YT-16 significantly enhanced T cell anti-tumor activity by blocking PD-1–PD-L1 interactions.