Evaluation of  biological activity of frog skin-derived peptide, ranatensin — ASN Events
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  3. Evaluation of  biological activity of frog skin-derived peptide, ranatensin

Evaluation of  biological activity of frog skin-derived peptide, ranatensin (#187)

Liliana Mrs. Palczynska 1 , Urszula Piotrowska 2 , Agnieszka Kowalczyk 1 , Attila Borics 3 , Kamil Koronowski 1 , Wojciech Kleniewski 1 , Karol Zakrzewski 1 , Edina Szűcs 3 4 , David Gombos 3 , Adriano Mollica 5 , Sandor Benyhe 3 , Marcin Sobczak 6 , Patrycja Kleczkowska 1
  1. Department of Pharmacodynamics and Pathophysiology, Centre for Preclinical Research and Technology (CePT), Medical University of Warsaw, Warsaw, Masovian, Poland
  2. Department of Medical Sciences, Faculty of Health Science and Physical Education, Kazimierz Pulaski University of Technology and Humanities in Radom, Radom, Poland
  3. Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary
  4. Doctoral School of Theoretical Medicine, University of Szeged, Szeged, Hungary
  5. Dipartimento di Farmacia, Università di Chieti-Pescara“G. d’Annunzio”, Chieti, Italy
  6. Department of Biomaterials Chemistry, Chair of Inorganic and Analytical Chemistry, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Warsaw, Poland

Despite great development in antinociceptive medicines, pain is still one of the most common reasons why patients seek medical care. Moreover, it is usually associated with other disease that affects our body (e.g. cancer, inflammation, bacterial infection). Nowadays, patients are taking more than one medicine at the same time which in turn, can increase adverse events or addiction rate. Therefore, new findings are needed. Recently, the focus has been on bombesin-related peptides.

Bombesin (BBS) is one of the active peptides purified from amphibian skin. This peptide together with a number of its family members was found active in mammals and its pharmacological effect extends into various physiological aspects.

Considering abovementioned, in our study we would like to present one of the bombesin-related peptides, which is ranatensin (RAN) - an undecapeptide with the amino acid sequence of Pyr-Val-Pro-Gln-Trp-Ala-Val-Gly-His-Phe-Met.NH2 as a highly potent natural compound with multitherapeutic potency. Indeed, RAN – located in cutaneous granular gland of frog skin but also widely distributed in brain tissue – was found to posses pain-relieving activity. In fact, intracerebroventricular (i.c.v.) administration of ranatensin resulted in a dose-dependent antinociceptive reponse after thermal and mechanical noxious stimuli application. Moreover, this beneficial effect was suggested independent of the opioidergic system as naloxone failed to antagonize the response in vivo. This action was also confirmed in in vitro binding studies as RAN was characterized by lack of interactions with MOR receptors.

Numerous peptides secreted by the skin of Amazonian frogs are known for their characterized ability to inhibit the growth and replication of bacteria. Thus, herein we also focused on the evaluation of other ranatensin’s exerted activities such as antibacterial and anti-hemolysis activities.

 

 

Acknowledgment: This study was partially carried out with the use of CePT infrastructure financed by the European Union – the European Regional Development Fund within the Operational Programme ‘Innovative economy’ for 2007–2013.